Fitting a curve onto the standards results of an ELISA is not straight-forward. However, Excel can be coerced to perform the curve fitting using the Solver addin. In this blog post I collect some online resources on curve fitting.
Findlay JW, Dillard RF. Appropriate calibration curve fitting in ligand binding assays. AAPS J. 2007;9:E260-E267.
Liao JJZ & Liu R. Re-parameterization of ﬁve-parameter logistic function. J. Chemometrics 2009;23:248–253.
Gottschalk PG, Dunn JR. The five-parameter logistic: a characterization and comparison with the four-parameter logistic. Anal Biochem. 2005;343:54-65.
MiraiBio, a company that specializes in curve-fitting, has some very good blog articles on this topic:
- Bioassay Analysis with the Five Parameter Logistic (5-PL) Non-Linear Regression Curve-Fitting Model
- The importance of weighting with the 4PL and 5PL
Curve-fitting with Excel
There is a semi-automated and an automated spreadsheet for Excel by Andreas Swart.
Curve-fitting with the Toolbox
I am hoping to implement a 4-parameter or 5-parameter logistic regression function into the next generation of the XL Toolbox.